AAV9 Luciferase

AAV9 Luciferase
Artikelnummer
BPS78459
Verpackungseinheit
50 µl x 2
Hersteller
BPS Bioscience

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Applications: Use as a positive control for transductionOptimize transduction assays and track protein expression over time

Description: Adeno-Associated Virus serotype 9 (AAV9) is one of the most promising serotypes for gene therapy applications. AAV9 transduces a wide range of tissue types, including cardiac and skeletal muscle, liver, pancreas, and eye tissue. AAV8 and AAV9 have recently been used to correct disease-causing mutations and improve muscle function in mouse models of Duchenne muscular dystrophy. AAV9 has significantly lower seroprevalence in the human population than other AAV serotypes, making AAV9 a desirable candidate for therapeutic applications.         These AAV particles constitutively express the firefly (Photinus pyralis) luciferase gene under the control of a CMV promoter. AAV transduction efficiency can easily be verified by measurement of luciferase activity.

Formulation: AAV was produced in HEK293-AAV cells and is supplied in PBS-MK (PBS Magnesium-Potassium) buffer containing 0.01% Pluronic F68.

Purification: The purity of the AAV particles was confirmed to be greater than 90% by staining with One-Step Lumitein™ UV Protein Gel Stain (Biotium #21005-1L). The purity will vary with each lot; the exact value will be provided with each shipment.

Storage Stability: AAV is shipped with dry ice. For long-term storage, it is recommended to store AAV at -80°C. 

Supplied As: Two vials (50 µl x 2) of AAV at a titer ≥1 x 1012 TU/ml. The titer is determined by qPCR and will vary with each lot; the exact value will be provided with each shipment.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: BSL-1
Mehr Informationen
Artikelnummer BPS78459
Hersteller BPS Bioscience
Hersteller Artikelnummer 78459
Verpackungseinheit 50 µl x 2
Mengeneinheit PAK
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