CloneID: AS30SS_Hu6
Antigen Long Description: The original antibody was generated by immunizing Balb/c and Trianni mice with human beta-dystroglycan. Humanized version was generated by grafting CDRs of parental mouse antibody AS30 onto human framework regions.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: Q14118; Q62165
Specificity Statement: This antibody recognizes human and mouse beta dystroglycan (β-DAG). The binding of this antibody to beta dystroglycan does not affect its binding to alpha dystroglycan. the Beta-dystroglycan is a transmembrane protein that plays an important role in connecting the extracellular matrix to the cytoskeleton. It plays an important role in linking dystrophin to the laminin-binding alpha-dystroglycan. It acts as a cell adhesion receptor in both muscle and non-muscle tissues. It also functions in cell adhesion-mediated signaling and implicated in cell polarity.
Application Notes (Clone): The binding specificity of this antibody for human and mouse beta dystroglycan (beta-DG) was confirmed using ELISA. This antibody is capable of immunoprecipitating both alpha-DG and beta-DG from C2C12 cell lysates, suggesting that the binding epitope of this antibody on beta dystroglycan is different from that of alpha dystroglycan. This antibody can also be used for the identification human beta-DG, mouse beta-DG and recombinant beta dystroglycan in a western blot. This antibody was also used for the immunofluorescent staining of unfixed human and mouse muscle tissues. This antibody was used in combination with anti-laminin-2 antibody C3_Hu10 to generate a duobody called TBTI (US11254737).
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