Anti-CD16a Antibody, PE-Labeled

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Application: Useful for studying expression of CD16a on the cell surface by flow cytometry.

Assay Conditions: One million CD16a CHO cells (BPS Bioscience #78332) were blocked and stained with 1 µg of anti-CD16a antibody, PE-labeled (BPS Bioscience #101978) for 30 minutes on ice, washed three times, and analyzed by flow cytometry. CD16a CHO recombinant cells (green) were compared to parental CHO-K1 cells (blue). The Y-axis represents the cell count while the X-axis shows PE intensity.

Background: CD16a, also known as Fc Gamma Receptor 3A (FcGRIIIA; FcγRIIIA), is a low/intermediate affinity receptor for polyvalent immune-complexed IgG. It is involved in phagocytosis, antibody-dependent cytotoxicity, and clearance of immune complexes. The human FcGR3A displays a dimorphism at residue 158. The allele V158 encodes a higher Fc affinity receptor variant while the allele F158 encodes a lower Fc affinity receptor variant. FcGR3A (CD16a) plays a role in the activation of natural killer (NK) cells, and it is extensively used as a cell marker. Its expression has also been observed at the surface of T cells in patients with chronic viral infections (such as COVID-19). Binding to CD16a can potentiate the efficacy of therapeutic antibodies used to treat solid tumors. In addition, CD16a is a biomarker in hematopoietic cancers and an interesting therapeutic target.

Cross Reactivity: This antibody recognizes human CD16a. It has not been tested on other species.

Description: This human anti-CD16a antibody is a purified recombinant antibody, which is labeled with R-Phycoerythrin (PE). This antibody has been tested by flow cytometry for binding to human CD16a on the surface of the CD16a CHO Recombinant Cell Line (BPS Bioscience #78332).

Format: Aqueous buffer solution.

Formulation: 8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, 0.09% Sodium Azide, 0.2% BSA, and up to 20% glycerol. May contain a protein stabilizer.

Host Cell Line: HEK293

Purification: Protein A affinity chromatography from HEK293 supernatants.

Purity: ≥90%

Storage Stability: At least 6 months at 4°C. Protect from prolonged exposure to light. DO NOT FREEZE. The antibody solution should be stored undiluted between 2°C and 8°C. 

Uniprot: P08637

Warnings: Avoid freeze/thaw cycles

Biosafety Level: Not applicable (BSL-1)

• Artikelnummer: BPS101978-1
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 101978-1
• Verpackungseinheit: 25 µg
• Mengeneinheit: STK
• Klonalität: Monoclonal
• Isotyp: Human IgG1
• Wirt: Human
• Konjugat: Conjugated, PE (Phycoerythrin)