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Amino Acid Sequence: MALPVTALLLPLALLLHAARP DIQLTQSPASLAVSLGQRATISCKASQSVDYDGDSYLNWYQQIPGQPPKLLIYDASNLVS GIPPRFSGSGSGTDFTLNIHPVEKVDAATYHCQQSTEDPWTFGGGTKLEIKGGGGSGGGG SGGGGSQVQLQQSGAELVRPGSSVKISCKASGYAFSSYWMNWVKQRPGQGLEWIGQIWPG DGDTNYNGKFKGKATLTADESSSTAYMQLSSLASEDSAVYFCARRETTTVGRYYYAMDYW GQGTTVTVSSGGGGSDIKLQQSGAELARPGASVKMSCKTSGYTFTRYTMHWVKQRPGQGL EWIGYINPSRGYTNYNQKFKDKATLTTDKSSSTAYMQLSSLTSEDSAVYYCARYYDDHYC LDYWGQGTTLTVSSVEGGSGGSGGSGGSGGVDDIQLTQSPAIMSASPGEKVTMTCRASSS VSYMNWYQQKSGTSPKRWIYDTSKVASGVPYRFSGSGSGTSYSLTISSMEAEDAATYYCQ QWSSNPLTFGAGTKLELKHHHHHH
Application: This product is for research use only. It is not suitable for human diagnostic or therapeutic use. The anti-CD19-Anti-CD3-His-Tag can be used as a reference BiTE for studying CD19+ cancer cell-mediated T cell activation, using either primary T cells or reporter cell lines such as Jurkat/NFAT-luc (BPS #60621) or Jurkat/IL2-luc (BPS #60481).
Background: Blinatumomab is a biopharmaceutical drug used as a second-line treatment for Philadelphia chromosome-negative relapsed or refractory acute lymphoblastic leukemia. It binds to CD19 on cancer cells (e.g. Raji cells) and CD3 on T cells simultaneously, thus bringing T lymphocytes closer to the cancer cells. The binding event potentiates unstimulated T cells and induces direct cytotoxicity against CD19+ cancer cells
Description: Anti-CD19-Anti-CD3-His-Tag is a purified recombinant human bispecific T cell Engager (BiTE) based on Amgen's Blinatumomab. This BiTE has been tested for specific activity in both ELISA binding assay to CD19-biotin and functional reporter assays using Jurkat/NFAT-luc or Jurkat/IL2-Luc reporter cell lines in the presence of CD19+ Raji cells.
Format: Aqueous Buffer Solution
Formulation: 8 mM phosphate, 110 mM NaCl, 2.2 mM KCl, pH 7.4, and 20% glycerol
Purification: Ni-NTA affinity purification of the His-tag protein from HEK293 cells.
Storage Stability: Stable for at least 12 months at -80°C. Avoid freeze/thaw cycles.
Supplied As: Aqueous buffer solution.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)