CloneID: MB19-1
Antigen Long Description: This antibody was raised by immunising CD19-deficient mice with a cell line of mCD19+ mouse pre-B cells.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This is a chimeric dog antibody, based on the original mouse IgA format, created for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P25918
Specificity Statement: This antibody recognizes murine MB19-1 (CD19), a transmembrane protein present in B lymphocytes that regulates its activation. It is required for proper differentiation of B-1 cells.
Application Notes (Clone): This clone can be used to detect and analyze the presence of the CD19 transmembrane protein. The specificity towards the protein has been confirmed using ELISA and immunofluorescence (Sato et al., 1996; PMID: 8906812). Additionally, the MB19-1 antibody has been used in several flow cytometry assays. One group used this antibody to determine the differences in CD19 distribution between cells of young and old mice (Yamamoto et al., 2018; PMID: 29625072). Another group used it to differentiate eosinophils from other leukocytes (Vanella et al., 2017; PMID: 27043413). A final group used flow cytometry to determine the fraction of B cells cells in a sample with this antibody (Bedaret et al., 2009; PMID: 19907079).
Deutsch
English
