Anti-CD8 Antibody, PE-Labeled

Quote the promo code SZANTIBODY when ordering to receive a 10% discount on this antibody, from October 1st to December 31st 2024. No additional discounts during the promo.

Products from BPS Bioscience require a minimum order value above 400€

Application: Useful for studying expression of CD8 on the cell surface by flow cytometry.

Assay Conditions: One million PBMC cells (BPS Bioscience #79059) were blocked and stained with 1 µg of anti-CD8 antibody, PE-labeled (BPS Bioscience #102011) or PE Human IgG1 Isotype Control (BioLegend #403503) for 30 minutes on ice, washed three times, and analyzed by flow cytometry. Non-lymphoid cells were excluded from the analysis based on side scatter and forward scatter signals. Y-axis represents the cell count while X-axis indicates PE intensity.

Background: CD8 is a co-receptor for the TCR (T cell receptor) in T cells, binding to MHC (major histocompatibility complex) class I proteins. CD8 is a typical marker of cytotoxic T cells and is involved in signaling. CD8 has two isoforms, a and b. CD8b recruits Lck (lymphocyte-specific protein tyrosine kinase) to the TCR-CD3 complex, and Lck phosphorylates multiple proteins involved in activation of cytotoxic T lymphocytes. It is thus critical for the lysis of cancer cells.

Cross Reactivity: This antibody recognizes human CD8. It has not been tested on other species.

Description: This anti-CD8 antibody is a purified recombinant antibody, which is labeled with R-Phycoerythrin (PE). This antibody has been tested by flow cytometry for specific binding to human CD8 on the surface of PBMCs (BPS Bioscience #79059).

Format: Aqueous buffer solution.

Formulation: 8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, 0.09% Sodium Azide, 0.2% BSA, and up to 20% glycerol. May contain a protein stabilizer.

Host Cell Line: HEK293

Purification: Protein A affinity chromatography from HEK293 supernatants.

Purity: ≥90%

Storage Stability: At least 6 months at 4°C. Protect from prolonged exposure to light. DO NOT FREEZE. The antibody solution should be stored undiluted between 2°C and 8°C. 

Warnings: Do not freeze. Protect from light.

Biosafety Level: Not applicable (BSL-1)

• Artikelnummer: BPS102011-1
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 102011-1
• Verpackungseinheit: 25 µg
• Mengeneinheit: STK
• Klonalität: Monoclonal
• Isotyp: IgG1
• Wirt: Human
• Konjugat: Conjugated, PE (Phycoerythrin)