CloneID: 2.43
Antigen Long Description: This antibody was generated by immunizing a Lewis rat with i.v. 3 times at 2-wk intervals with 15 * 10^6 L3 cloned cytolytic T cells. Three days after the third immunization, the rat spleen cells were fused with either P3 or SP2/0 cells using polyethylene glycol.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P01731
Specificity Statement: This antibody is specific for the lyt-2 protein of mice (it is psecific to the Lyt2.2 variant which is expressed in the mouse strains BALB/c and C57BL/6 (B/6)). Lyt-2 is an integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex.
Application Notes (Clone): This antibody was used for an immunofluorescence experiment on mouse thymocytes and mouse spleen cells (Sarmiento et al, 1980; pmid:6159417). Immunoprecipitation was preformed on NP-40 extract of 126I surfae labeled L3 cells using this antibody (Sarmiento et al, 1980; pmid:6159417). This antibody was used to try and block the cytolytic activity from L3 against P-185. It only blocks the receptor-mediated but not lectin mediated cytolysis by L3 (Dialynas et al, 1981; pmid:6788878). Flow cytometry was preformed with this antibody on washed cells from the lymph node, spleen, thymus and peripheral blood from mice (Rodriguez and Sriram, 1988; pmid:2966203). This antibody was used to stain mouse spleen cells for use in flow cytometry (Mielke et al, 1988; pmid:2969373).
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