CloneID: 4396
Heavy Chain modification: Fc Silent™
Antigen Long Description: The original antibody was generated by immunizing mice with purified human brain-derived GM1-Aβ.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This is a reformatted mouse IgG2a Fc Silent™ antibody, based on the original mouse IgM format, created for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Specificity Statement: This antibody is specific for human GM1-Aβ.
Application Notes (Clone): The original mouse IgM antibody was developed and tested to specifically recognize Aβ bound to GM1 (GM1-Aβ). A subsequent version, 4396C, an IgG2a subtype chimeric antibody, was generated and evaluated. The chimeric antibody retained its specificity for GM1-Aβ, as demonstrated by electron microscopy (EM), where it specifically labeled GM1-containing liposomes incubated with Aβ; Western blotting (WB), which confirmed the antibody’s specificity for GM1-Aβ; a Thioflavin T (ThT) assay, which showed that the antibody inhibited amyloid fibril formation in a dose-dependent manner when incubated with GM1-containing liposomes and Aβ; and immunoelectron microscopy (IM), where it effectively bound to newly formed amyloid fibrils in the presence of synthetic Aβ and GM1-containing liposomes (US7339035B2). The chimeric antibody was assessed in three polymerization systems: (1) polymerization initiated by adding Aβ fibril fragment, (2) Aβ40 polymerization initiated from GAβ40, and (3) Aβ42 polymerization initiated from GAβ42. It exhibited suppression of the Aβ extension (US7741448B2).
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