Anti-IL-13 (6A1)

Anti-IL-13 [6A1], Recombinant, IgG1 kappa, Mouse

CloneID: 6A1

Antigen Long Description: The original antibody was generate by immunizing SJL mice with E. Coli-produced recombinant human IL-13.

Buffer Composition: PBS with 0.02% Proclin 300.

Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7

Uniprot Accession No.: P35225

Specificity Statement: This antibody is specific for human IL-13. It cross-reacts with cynomolgus monkey IL-13.

Application Notes (Clone): The original format of this antibody (mouse IgG1) showed binding activity against E.coli-expressed recombinant human IL-13 in a sandwich ELISA with an EC₅₀ of 0.049 and 0.039 µg/ml for human and cynomolgus IL-13. The antibody also exhibited potent binding and neutralization activities against various forms of IL-13. In inhibition assays, the antibody showed IC₅₀ values of 0.165 µg/ml for blocking human IL-13 binding to IL-13Rα1 and 0.056 µg/ml for IL-13 binding to IL-13Rα2. Moreover, it demonstrated strong neutralization capabilities with ND₅₀ (neutralizing dose) values of 0.0783 µg/ml for human IL-13 and 0.04 µg/ml for cynomolgus IL-13, indicating its efficacy in inhibiting IL-13-induced TF-1 cell proliferation (where the ND₅₀ value is the concentration of monoclonal antibody required to reduce TF-1 cell proliferation by 50%, in response to a set concentration of IL-13). Furthermore, the antibody effectively neutralized mammalian-expressed human IL-13 with an ND 50 value of 0.037 µg/ml and a recombinant Q130 human IL-13 variant with an ND₅₀ value of 0.110 µg/ml. The binding affinity of the antibody was measured by SPR and found to be 32, 270, and 61.2 pM for det-1 tagged human IL-13, human IL-13, and cynomolgus IL-13, respectively (EP1802658B1).

• Artikelnummer: ABAAb04494-1.1
• Hersteller: Absolute Antibody
• Hersteller Artikelnummer: Ab04494-1.1
• Verpackungseinheit: 100 μg
• Mengeneinheit: STK
• Reaktivität: Human, Cynomolgus Monkey (Primate)
• Klonalität: Recombinant
• Methode: ELISA, Neutralization, Super-Resolution Microscopy, Inhibition
• Isotyp: IgG1 kappa
• Wirt: Mouse