CloneID: G8
Heavy Chain modification: Fc Silent™
Antigen Long Description: The original antibody was isolated from a human antibody phage display library by bio-panning against human LAG-3.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This is a reformatted human IgG1 Fc Silent™ antibody, based on the original human IgG1 format, created for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P18627
Specificity Statement: The antibody binds to human and rhesus macaque LAG-3.
Application Notes (Clone): The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody was able to bind LAG-3 expressed at the surface of HEK-293 cells was evaluated by flow cytometry. The antibody showed binding to activated T cells. The antibody bound to cells expressing rhesus LAG-3 on transiently transfected HEK-293 cells. The affinity of the antibody to human LAG-3 was measured using Bio-Layer Interferometry (Kd = 0.70 nM). The antibody showed inhibitory capacity of the binding of LAG-3 to MHC class II in a dose dependent manner, and was able to completely block binding of LAG-3 to MHC-II at high concentrations (IC50 = 12 nM). The antibody could block the binding of LAG-3 to MHC class II-expressing Daudi cells in a dose-dependent manner by flow cytometry analysis. The antibody was able reverse exhaustion and restore the activity of T cells to secrete IL-2 and IFN-γ upon restimulation at high doses (US20190040136A1).
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