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Products from BPS Bioscience require a minimum order value above 400€Application: Useful for the inhibition of OX40:OX40L interactions.
Assay Conditions: Reaction done according to OX40[biotin]-OX40L assay kit (Catalog #72045). Buffer is PBS + 0.1% BSA. Blocking buffer is Superblock + 0.05% Tween-20. Coat protein was added ON at 4 degrees. Inhibitor and then biotinylated ligand added for 2 hours at room temperature. Strep-HRP added for 1 hour at room temperature.
Description: Recombinant human Anti-OX40competitive antibody recognizing the OX40binding region. This antibody has not beentested for cross-reactivity with any otherspecies.
Format: Aqueous buffer solution
Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, pH 7.4, and 20% glycerol
Storage Stability: Stable for at least 12 months at -80°C.
Uniprot: P23510
Warnings: Protect from light. Avoid freeze/thaw cycles. Store diluted antibody with carrierprotein (BSA 0.1 - 0.5%) at 4°C, stable for 2 weeks.
Biosafety Level: Not applicable (BSL-1)
References: 1. Garrison, K., et al., Cancer Immunology. 2012 April; 61(4): 511-521.2. Bulliard, Y., et al., Immunology & Cell Biology. 2014 Apr; 92: 475-480.