Anti-PKM2 Antibody Picoband™ (monoclonal, 11I4C3)

Clone Number: 11I4C3

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human PKM2, different from the related mouse sequence by five amino acids, and from the related rat sequence by four amino acids.

Concentration: Adding 0.2 ml of distilled water =f 500 μg/ml.

Format: Lyophilized

Storage buffer: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Additional info: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. Background: PKM (Pyruvate Kinase, Muscle), also known as PK3 or PKM2, is an enzyme that in humans is encoded by the PKM gene. The activity of pyruvate kinase subtype M2 is increased by fructose 1, 6-bisphosphate (Fru-1, 6-P2). By in situ hybridization, Popescu and Cheng (1990) mapped the THBP1 gene to 15q24-q25. Ashizawa et al. (1991) manipulated the intracellular Fru-1, 6-P2 concentration in several mammalian cell lines, including human, by varying the glucose concentration in the media. Using a novel proteomic screen for phosphotyrosine-binding proteins, Christofk et al. (2008) observed that PKM2 binds directly and selectively to tyrosine-phosphorylated peptides. Subcellular Localization: Tissue Specificity:

• Artikelnummer: SANMON23841
• Hersteller: Sanbio / Monosan
• Hersteller Artikelnummer: MON23841
• Verpackungseinheit: 100μg
• Mengeneinheit: STK
• Reaktivität: Human
• Klonalität: Monoclonal
• Methode: Immunofluorescence, Western Blotting, Flow Cytometry, Immunohistochemistry, Immunocytochemistry
• Isotyp: IgG2b
• Wirt: Mouse
• Konjugat: Unconjugated