CloneID: 2B11
Heavy Chain modification: Fc Silent™
Antigen Long Description: The original antibody was selected from a synthetic human scFv library through biopanning against recombinant PspA.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This is a reformatted human IgG1 Fc Silent Fc Silent™ antibody, based on the original human scFv format, created for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: A0A1M4NIX2
Specificity Statement: This antibody is specific for the Streptococcus pneumoniae PspA. The epitope on PspA was defined to residues 231-242 by mutational analysis and supported by molecular docking analysis.
Application Notes (Clone): The dissociation constant (Kd) of the original human scFv version of this antibody was 5.3 ± 2.3 nM as measured in a biolayer interferometry assay. This antibody detected PspA in immunoblots; it exhibited a dose-dependent recognition of recombinant PspA with no cross-reactivity towards other tested pneumococcal antigens and also detected endogenous PspA from pneumococcal lysates (Jang et al., 2017; PMID: 27845043).
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