Anti-PVRIG Neutralizing Antibody

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Application: Useful for binding and neutralization of PVRIG in ELISA and in cellular assays.

Assay Conditions: The antibody was validated by measuring its binding to a PVRIG antigen in ELISA. PVRIG protein (BPS Bioscience #101712) was coated onto a 96-well plate overnight at 4°C (50 µl/well at a concentration of 2 µg/ml in PBS). The plate was washed 3 times with Immuno Buffer 1 (BPS Bioscience #79311) and blocked using 100 µl of Blocking Buffer 2 (BPS Bioscience #79728) for 1 hour at Room Temperature (RT). After removing the blocking buffer, 50 µl/well of serially diluted CD112R (PVRIG), Fc-Fusion, Avi-Tag, Biotin-Labeled (Human) Recombinant (BPS Bioscience #79270) in Blocking Buffer 2, was added for 1 hour at RT. After 3 washes, the plate was incubated with Streptavidin-HRP (BPS Bioscience #79742), washed again, and colorimetric HRP substrate was added. The reaction was stopped, and absorbance was read at λ=450 nm. The Blank value was subtracted from all values.

Background: PVRIG, also known as Poliovirus receptor related immunoglobulin domain containing protein or CD112R, is a natural killer (NK) cell immune checkpoint receptor, and it is also found in CD8⁺ T cells. PVRIG works as an inhibitory receptor and recognizes CD112 (also known as poliovirus receptor-related 2, PVRL2 or nectin-2) as its ligand. This binding results in lower responses of NK and T cells towards cancer cells. In T cells it inhibits the nuclear factor of activated T cells (NFAT). Blocking the interaction between PVRIG and its ligand, for instance with an antibody directed towards PVRIG, represents a novel strategy in checkpoint blockade immunotherapy.

Description: This anti-PVRIG (Poliovirus receptor related immunoglobulin domain containing) antibody is a purified, recombinant human monoclonal antibody which recognizes human PVRIG. This recombinant antibody has been tested for specific binding to purified recombinant human PVRIG in an ELISA binding assay.

Format: Aqueous buffer solution.

Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

Purity: ≥90%

Storage Stability: At least 12 months at -80°C.

Uniprot: Q6DKI7

Warnings: Avoid freeze/thaw cycles

Biosafety Level: Not applicable (BSL-1)

• Artikelnummer: BPS101712-1
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 101712-1
• Verpackungseinheit: 50 µg
• Mengeneinheit: STK
• Klonalität: Monoclonal
• Methode: ELISA
• Isotyp: IgG1
• Konjugat: Unconjugated