Anti-Streptavidin antibodies have been widely used as amplifying reagents in immunohistochemistry, in situ hybridization, microarray assays, ELISAs, blots, and many other applications. Our antibodies to streptavidin are produced in goats using our highly purified streptavidin and isolated by affinity chromatography. Anti-Streptavidin does not bind avidin and Anti-Avidin does not recognize streptavidin. These antibodies provide opportunities to significantly amplify signals in many applications.