APAF1 antibody

Application Note: WB: 0.25-0.5 μg/ml. FACS: 1-3 μg/1x10⁶ cells. ELISA: 0.1-0.5 μg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Calculated MW: 142

Form: Liquid

Buffer (with preservative): 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, no preservatives.

Concentration: 0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Background: This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot ID: O14727

Antigen Species: Human

Immunogen: E.coli-derived human APAF1 recombinant protein (Position: M1-E1248)

Purification: Purified by antigen-affinity chromatography

Conjugation: Unconjugated

Full Name: apoptotic peptidase activating factor 1

• Artikelnummer: GTX04330-100
• Hersteller: GeneTex
• Hersteller Artikelnummer: GTX04330-100
• Green Labware: Nein
• Verpackungseinheit: 100 μg
• Mengeneinheit: STK
• Reaktivität: Human
• Klonalität: Polyclonal
• Methode: Western Blotting, ELISA, Flow Cytometry
• Isotyp: IgG
• Human Gene ID: 317
• Wirt: Rabbit