Blood Urea Nitrogen Detection Kit

Quantitative colorimetric measurement of urea nitrogen

Target: Blood Urea Nitrogen

Product Type: Detection Kits

Trademark: StressXpress®

Detection Method: Colorimetric Assay

Assay Type: Direct Quantitative Assay

Utility: Colorimetric assay used to quantitatively measure urea nitrogen in a variety of samples.

Precision: Intra-Assay Precision (Within Run Precision)To determine Intra-Assay Precision, three samples of known concentration were assayed at different times, with different operators. The intra-assay coefficient of variation of the BUN within assays was determined to be <10%.Inter-Assay Precision (Between Run Precision)To determine Inter-Assay Precision, three samples of known concentration were assayed at different times, with different operators. The inter-assay coefficient of variation of the BUN between assays was determined to be <20%.

Sample Type(s): Serum,Plasma,Urine,Saliva,Tissue Culture Media

Number of Samples: 88 samples in duplicate

Incubation Time: 30 Minutes

Sensitivity: 0.042 mg/dl

Assay Range: 0.156 - 10 mg/dl

Scientific Background: Urea is a by-product of protein metabolism by the liver, and is therefore removed from the blood by the kidneys. Urea freely filters through the glomerulous, but is reabsorbed by the renal tubules in a flow-dependent fashion. The higher the flow rate, the greater amount of urea nitrogen is cleared from circulation and eliminated through the kidneys. As a result, the level of circulating urea nitrogen, along with serum creatinine, serves as a primary measure of kidney function. Normal adult Blood Urea Nitrogen (BUN) levels should be between 7 and 21 mg urea nitrogen per 100 mL blood (mg/dL) (1). Azotemia, poor kidney function, will cause elevated BUN levels (≥ 50 mg/dL) and is associated with acute kidney failure or injury, severe acute pancreatitis, congestive heart failure or gastrointestinal bleeding (2-5). Azotemia also can occur with dehydration, as a result of alcohol abuse, or high protein diets. Lower than expected BUN levels are usually not clinically predictive, but are primarily associated with liver disease or malnutrition, including malabsorption and low protein diets (6). Urine and saliva are considered to be acceptable non-invasive samples for measurement of urea nitrogen (7).Serum creatinine is another metabolic waste product freely filtered by the glumerulous, but does not undergo tubular reabsorption. Its steady rate of elimination is frequently used to generate an index or ratio with BUN values for normalized evaluations. Creatinine Urinary Detection Kit (SKT-200) and Creatinine Serum Detection Kit (SKT-217) are also available from StressMarq Biosciences.

References: 1. Laboratory reference values. Urea nitrogen (BUN). Rochester, Minn.: Mayo Foundation for Medical Education and Research; Nov. 2010.2. Waiker, SS and JV Bonventre. (2008) Nephron Clin. Pract. 109:c192-c197. 3. Al Mofleh, IA. World J. Gastroent. (2008) Congestive heart failure. 14(5):675-684. 4. Iglesiase, J. et al. (2006) Nephrol. Dial. Transplant. 21:3458-3465.5. Mayo Clinic. “Blood urea nitrogen (BUN) tests.” http://www.mayoclinic.com/health/blood-urea-nitrogen/MY00373/DSECTION=results6. Lum, G and S Leal-Khouri. (1989) Clin. Chem. 35(4):639-640. 7. Akai, T, et al. (1983) Clin. Chem. 1983. 29(10):1825-1827.

Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• Artikelnummer: STRSKT-213-192
• Hersteller: Stressmarq Biosciences
• Hersteller Artikelnummer: SKT-213-192
• Green Labware: Nein
• Verpackungseinheit: 96 well x2
• Mengeneinheit: PAK
• Reaktivität: Various species