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BrdU Cell Proliferation Assay Kit
BrdU Cell Proliferation Assay Kit, N/A
Artikelnummer
EXAX1327K2
Verpackungseinheit
1000 Tests
Hersteller
Exalpha Biologicals Inc
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Label: BrdU,HRP
Background: The BrdU Cell Proliferation Assay Kit is a non-isotopic enzyme immunoassay for the quantification of DNA synthesis and cell proliferation. Evaluation of cell cycle progression is essential for investigations in many scientific fields. Measurement of [3H] thymidine incorporation as cells enter S phase has long been the traditional method for the detection of cell proliferation. Subsequent quantification of [3H] thymidine is performed by scintillation counting or autoradiography. This technology is slow, labor intensive and has several limitations including the handling and disposal of radioisotopes and the necessity of expensive equipment. A well-established alternative to [3H] thymidine uptake has been demonstrated by numerous investigators. In these methods bromodeoxyuridine (BrdU), a thymidine analog, replaces [3H] thymidine. BrdU is incorporated into newly synthesized DNA strands of actively proliferating cells. Following partial denaturation of double stranded DNA, BrdU is detected immunochemically allowing the assessment of the population of cells which are actively synthesizing DNA. Exalpha Biologicals BrdU Cell Proliferation Assay Kit involves incorporation of BrdU Reagent into cells cultured in microtiter plates using the cell layer as the solid phase. The resultant assay is, rapid, easy to perform and applicable to high sample throughput. In addition to evaluation of cell proliferation, information such as cell number, morphology and analysis of cellular antigens can be obtained from a single culture.
References: 1. Hawker JR Jr., 'Chemiluminescence-based BrdU ELISA to measure DNA synthesis.' J Immunol Methods. 2003 Mar 1;274(1-2):77-82.2. Ang, L.P.K., et al. 'Development of a conjunctival epithelial equivalent with improved proliferative properties using a multistep serum-free culture system.' Investigative Ophthalmology & Visual Science, 2004, 45, 1789-1795
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Background: The BrdU Cell Proliferation Assay Kit is a non-isotopic enzyme immunoassay for the quantification of DNA synthesis and cell proliferation. Evaluation of cell cycle progression is essential for investigations in many scientific fields. Measurement of [3H] thymidine incorporation as cells enter S phase has long been the traditional method for the detection of cell proliferation. Subsequent quantification of [3H] thymidine is performed by scintillation counting or autoradiography. This technology is slow, labor intensive and has several limitations including the handling and disposal of radioisotopes and the necessity of expensive equipment. A well-established alternative to [3H] thymidine uptake has been demonstrated by numerous investigators. In these methods bromodeoxyuridine (BrdU), a thymidine analog, replaces [3H] thymidine. BrdU is incorporated into newly synthesized DNA strands of actively proliferating cells. Following partial denaturation of double stranded DNA, BrdU is detected immunochemically allowing the assessment of the population of cells which are actively synthesizing DNA. Exalpha Biologicals BrdU Cell Proliferation Assay Kit involves incorporation of BrdU Reagent into cells cultured in microtiter plates using the cell layer as the solid phase. The resultant assay is, rapid, easy to perform and applicable to high sample throughput. In addition to evaluation of cell proliferation, information such as cell number, morphology and analysis of cellular antigens can be obtained from a single culture.
References: 1. Hawker JR Jr., 'Chemiluminescence-based BrdU ELISA to measure DNA synthesis.' J Immunol Methods. 2003 Mar 1;274(1-2):77-82.2. Ang, L.P.K., et al. 'Development of a conjunctival epithelial equivalent with improved proliferative properties using a multistep serum-free culture system.' Investigative Ophthalmology & Visual Science, 2004, 45, 1789-1795
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Immer für Sie da
Gernot Ensinger, M.Sc.
