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Encompassing Amino Acids: 1-1227
Description: Lachnospiraceae Cas12, also known as LpCpf1, Genbank Accession No. ATB19154, a.a 1-1227, with a C-terminal His-tag, expressed in an E. coli expression system. MW=130 kDa.
Format: Aqueous buffer solution
Formulation: 50 mM sodium phosphate, pH 7.5, 300 mM NaCl, 150 mM imidazole, 1 mM DTT 10% glycerol.
Genbank: ATB19154
Storage Stability: At least 6 months at -80°C.
Supplied As: Aqueous buffer solution.
Tags: C-terminal His-tag
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Zhou, P., et al., Nature. 2020; 579:270-289.
2. Stefano, S., et al., Biophysical Journal. 2020; 118 (3): 223A-224A.