Caspase3, His-Tag Recombinant

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Encompassing Amino Acids: 1 - 277 (end)

Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: Assayed using BPS's Homogeneous Caspase-3 Assay Kit, Cat. # 80700. Assay conditions: 10 mM HEPES, pH7.4, 10 mM EDTA, 0.05% Chaps, 5 mMDTT, and 20 µM Caspase 3 substrate.Incubate for 30 minutes at roomtemperature. Fluorescence intensity ismeasured at exc380/em500 nm.

Background: Caspase-3 is a member of the caspase (cysteine aspartate protease) family of proteins, and has been shown to be an executioner protein of apoptosis.

Description: Human caspase 3 (CASP3), also known as CPP32 and apopain, a.a. 1 - 277 (end) (GenBank Accession No. NM_004346, var alpha) with C-terminal His-tag, expressed in an E. coli expression system. Procaspase-3 is self-cleaved into two subunits, MW = 17 kDa and 13 kDa, to form the active heterotetrameric complex.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol and 3 mM DTT.

Genbank: NM_004346

Purity: ≥90%

Storage Stability: At least 6 months at -80°C.

Tags: C-terminal His-tag

Uniprot: P42574

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Lee, D., et al., J Biol Chem. 2000 May 26;275(21):16007-14
2. Suzuki, Y., et al., Proc Natl Acad Sci USA. 2001 Jul 17;98(15):866-7.

• Artikelnummer: BPS80500-1
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 80500-1
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK