CD200:CD200R1 (Biotinylated) Inhibitor Screening Chemiluminescence Assay Kit

Products from BPS Bioscience require a minimum order value above 400€

Applications: Screen small molecule inhibitors or antibodies that block CD200 binding to CD200R1.

Background: CD200 (Cluster of Differentiation 200) and its receptor, CD200R, are cell surface proteins that play a crucial role in immune regulation and tolerance. The CD200-CD200R pathway is primarily involved in inhibiting immune responses, helping to maintain immune homeostasis and prevent excessive inflammation. In many cancers, tumor cells overexpress CD200, which interacts with CD200R on immune cells, particularly myeloid cells like macrophages and dendritic cells. This interaction leads to the suppression of immune responses against the tumor, effectively promoting immune evasion. Neutralizing antibodies against CD200 or CD200R can block this interaction, preventing the inhibitory signal from being delivered to immune cells. As a result, the anti-tumor immune response is enhanced, leading to improved recognition and elimination of tumor cells by the immune system. The development of strategies targeting this complex holds great potential in cancer therapy.

Contraindications: The DMSO concentration in the final reaction should be ‰¤1%.

Description: The CD200:CD200R1 [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit is designed for screening and profiling molecules that block the binding between CD200 (cluster of differentiation 200) and CD200R1 (CD200 receptor 1). This kit comes in a convenient 96-well format, with enough recombinant human biotin-labeled CD200R1 (amino acids 29-265), human CD200 (amino acids 31-232), streptavidin-HRP, and assay buffer for 100 binding reactions.CD200 is coated onto a 96-well plate. After blocking, the plate is pre-incubated with an inhibitor or neutralizing antibody. After incubation with Biotin-CD200R1, the plate is washed and Streptavidin-HRP is added. After the  ELISA ECL substrate is added  the resulting signal can be measured using a chemiluminescence microplate reader. The chemiluminescence signal is proportional to the binding of CD200 to CD200R1.

Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Uniprot: P41217

Warnings: Avoid freeze/thaw cycles

Biosafety Level: Not applicable (BSL-1)

• Artikelnummer: BPS82285
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 82285
• Verpackungseinheit: 96 reactions
• Mengeneinheit: PAK