CD226, Fc fusion, Biotin-labeled (Human) HiP™ Recombinant

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Applications: Useful for studying protein binding and for screening small molecules and antibodies.

Assay Conditions: CD226, biotin binding to CD155, His-tag was confirmed according to the protocol for BPS Cat. 72052. Briefly, 6 ng of CD226-biotin was mixed with varying amounts of CD155, His-tag in CD226 assay buffer at a final volume of 10 µl and allowed to incubate for 1 hr at room temp. Nickle acceptor beads were added for 30 min, followed by Strep-conjugated acceptor beads for 30 min and reading alpha-counts.

Description: Human CD226 antigen also known as DNAX accessory molecule 1 (DNAM-1), GenBank Accession No. NM_006566, a.a. 19-247 fused to Fc region of human IgG1, this protein is enzymatically biotinylated using AviTag™ technology and expressed in a HEK293 cell expression system. MW = 54.7 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.

Format: Aqueous buffer solution

Formulation: 8 mM phosphate, pH 7.4,110 mM NaCl, 2.2 mM KCl, and 20%glycerol

Genbank: NM_006566

Storage Stability: At least 6 months at -80°C.

Tags: C-terminal Fc fusion (Human IgG1), Avi-Tag™

Uniprot: Q15762

Warnings: Avoid freeze/thaw cycles. Protein may be diluted to ≥100 µg/ml in PBS + glycerol and stored at -80°C.

Biosafety Level: Not applicable (BSL-1)

References: 1. Bottino, C., et al., J. Exp. Med. 2003; 198(4): 557-567.
2. Hafler, J., et al., Genes Immun. 2009; 10: 5-10.

• Artikelnummer: BPS71253-1
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 71253-1
• Green Labware: Nein
• Verpackungseinheit: 25 µg
• Mengeneinheit: STK