CD235a Positive Cell Isolation Kit

Products from BPS Bioscience require a minimum order value above 400€

Application: Isolate or deplete CD235a-expressing erythroid cells from a mixed population such as PBMCs.Positively selected cells, or CD235^- cells (depleted population), may be used for downstream applications such as genomic analysis, expression assays, protein isolation or flow cytometry.

Background: Erythroid cells are non-nucleated cells responsible for carrying oxygen from the lungs to tissues throughout the body. These cells are of interest in the study of fetal erythroblasts in maternal PBMCs and red blood cell disorders. Depletion of these cells from a mixed sample also allows to study other immune cells. CD235a, also known as glycophorin A, is a marker of erythroid precursors and erythroid cells. In PBMCs derived from healthy individuals, about 0.1-0.2% of the cells are CD235a⁺ cells.

Description: The CD235a Positive Cell Isolation Kit is designed to magnetically separate CD235a-expressing cells from a complex immune cell population. This kit is optimized for the isolation of CD235a positive cells from human peripheral blood mononuclear cells (PBMCs). Cells are incubated with the antibody:bead complex and placed on a magnet for quick and easy separation. When placed on the magnet, the CD235a-positive cells are immobilized along the side of the tube while undesired CD235a-negative cells remain in suspension and can be easily removed.

Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Supplied As: This kit provides enough reagents and materials for isolation from up to 1 x 10⁸ or 1 x 10⁹PBMCs. It is possible to use this kit for multiple isolations from smaller PBMC samples.

Warnings: Avoid freeze/thaw cycles

Biosafety Level: Not applicable (BSL-1)

• Artikelnummer: BPS78896-1
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 78896-1
• Verpackungseinheit: 1 x 10⁸ Cells
• Mengeneinheit: STK