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Applications: Useful for studying enzyme kinetics, substrate specificity, and screening inhibitors.
Assay Conditions: Assay was done in 50 µL assay buffer containing 40 mM Tris, pH 7.4, 5 mM MgCl2, 0.1 mg/ml BSA and 40 µM AMP for 20 min at 37°C. Inorganic phosphate produced was measured using Biomol Green (Enzo Life Science) reagent.
Description: Human CD73, also known as 5'- nucleotidase and Ecto-5'-nucleotidase, GenBank Accession No. NM_002526, a.a. 27-547 with C-terminal His-tag, expressed in a HEK293 cell expression system. MW = 58.6 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol
Genbank: NM_002526
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal His-tag
Uniprot: P21589
Warnings: Avoidfreeze/thaw cycles. Storing diluted protein isnot recommended, if necessary, use carrierprotein (BSA 0.1 - 0.5%).
Biosafety Level: Not applicable (BSL-1)
References: 1. Deaglio, S. et al., J. Exp. Med. 2007; 204(6): 1257-1265.
2. Thompson, L.F., et al., J. Exp. Med. 2004; 200(11): 1395-1405.