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Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Description: Human CEACAM, also known as Carcinoembryonic antigen-related cell adhesion molecule 1 or biliary glycoprotein, (GenBank Accession No. NM_ 001712), amino-acids 35-428, with C-terminal Avi-His-tag, MW = 47 kDa, expressed in HEK293 cells. This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.
Format: Aqueous buffer solution
Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Genbank: NM_ 001712
Host Cell Line: HEK293
Storage Stability: At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.
Tags: C-terminal Avi-His-tag
Uniprot: P13688
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Carvalho, F. et al., J.E.M. 206 (10), 2179 (2009).2. Hauck R. et al., FEBS. 478 (3), 260-266 (2000).