CENP-A Antibody, Clone 5A7-2E11: HRP

Mouse Anti-Human CENP-A Monoclonal IgG1

Target: CENPA

Conjugate: HRP

Product Type: Monoclonal

Clone Number: 5A7-2E11

Immunogen: Synthetic peptide corresponding to a portion of human CENP-A

Swiss-Prot: P49450

Purification: Protein G Purified

Storage Buffer: PBS, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated

Concentration: 1 mg/ml

Specificity: Detects ~18kDa. Recognizes Human CENP-A.

Cellular Localization: Nucleus,Chromosome,Centromere,Kinetochore

Scientific Background: A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. The Centromere Protein-A, CENP-A, is a Histone H3-like protein that contains a C-terminal H3-like domain, which is required for centromere localization of CENP-A, and an antigenic N-terminal domain. CENP-A, originally isolated from HeLa cells, is essential for kinetochore targeting of CENP-C. In the presence of DNA CENP-A forms an octa-meric complex with histones H4, H2A, H2B. CENP-A specifically localizes to active centromeres and is a component of specialized centromeric nucleosomes, on which kinetochores are assembled. CENP-A is essential for nucleosomal packaging of centromeric DNA at interphase and functions as a centromere formation marker on the chromosome.

References: 1. Rieder C.L., et al. (1998) Trends Cell Biol. 8: 310-318.2. Choo K.H. (2000) Trends Cell Biol. 10: 182-188.3. Muro Y., et al. (2000) Clin. Exp. Immunol. 120: 218-223.4. Howman E.V., et al. (2000) Proc. Natl. Acad. Sci. USA 97: 1148-1153.

Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• Artikelnummer: STRSMC-202D-HRP
• Hersteller: Stressmarq Biosciences
• Hersteller Artikelnummer: SMC-202D-HRP
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK
• Reaktivität: Human
• Klonalität: Monoclonal
• Methode: Immunofluorescence, Western Blotting, Immunocytochemistry
• Isotyp: IgG1
• Human Gene ID: 1058
• Wirt: Mouse