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Chemi-Verse™ AKT1 Kinase Assay Kit
Chemi-Verse™ AKT1 Kinase Assay Kit
Artikelnummer
BPS82218
Verpackungseinheit
96 reactions
Hersteller
BPS Bioscience
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Products from BPS Bioscience require a minimum order value above 400€
Applications: Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.
Background: AKT1, also known as Protein Kinase B Alpha (PKBα), is a serine/threonine kinase found in various cell types throughout the body, where it plays a critical role in regulating cell survival, insulin signaling, angiogenesis and tumor formation as well as controlling apoptosis. The signaling pathways involving AKT1 kinase are essential for the normal development and function of the nervous system. AKT1 has a broad number of targets and can thus regulate multiple pathways. Dysregulation of AKT1 contributes to a number of serious diseases, including cancer, diabetes, cardiovascular and neurological diseases. In cancer AKT1 is involved in tumor progression and drug resistance. For instance, the activating mutation E17K seems to be present in 7% of the estrogen-receptor positive metastatic breast cancers. The use of AKT inhibitors, such as capivasertib, alone or in combination, is currently under late-stage clinical trials and has been showing great promise for the treatment of cancer patients. A deep understanding of the mechanisms of action of AKT1 and how inhibition of AKT1 can lead to other drug resistance can be crucial to the development of new cancer therapies.
Contraindications: The final concentration of DMSO in the assay should not exceed 1%.
Description: The Chemi-Verse™ AKT1 Kinase Assay Kit is designed to measure AKT1 (protein kinase B) kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant AKT1 kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Uniprot: P31749
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
References: Smyth L., et al., 2020 Clin Cancer Res 26(15):3947-3957.
Hua H., et al., 2021 J Hematol Oncol 14:128.
Applications: Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.
Background: AKT1, also known as Protein Kinase B Alpha (PKBα), is a serine/threonine kinase found in various cell types throughout the body, where it plays a critical role in regulating cell survival, insulin signaling, angiogenesis and tumor formation as well as controlling apoptosis. The signaling pathways involving AKT1 kinase are essential for the normal development and function of the nervous system. AKT1 has a broad number of targets and can thus regulate multiple pathways. Dysregulation of AKT1 contributes to a number of serious diseases, including cancer, diabetes, cardiovascular and neurological diseases. In cancer AKT1 is involved in tumor progression and drug resistance. For instance, the activating mutation E17K seems to be present in 7% of the estrogen-receptor positive metastatic breast cancers. The use of AKT inhibitors, such as capivasertib, alone or in combination, is currently under late-stage clinical trials and has been showing great promise for the treatment of cancer patients. A deep understanding of the mechanisms of action of AKT1 and how inhibition of AKT1 can lead to other drug resistance can be crucial to the development of new cancer therapies.
Contraindications: The final concentration of DMSO in the assay should not exceed 1%.
Description: The Chemi-Verse™ AKT1 Kinase Assay Kit is designed to measure AKT1 (protein kinase B) kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant AKT1 kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Uniprot: P31749
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
References: Smyth L., et al., 2020 Clin Cancer Res 26(15):3947-3957.
Hua H., et al., 2021 J Hematol Oncol 14:128.
| Artikelnummer | BPS82218 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 82218 |
| Verpackungseinheit | 96 reactions |
| Mengeneinheit | PAK |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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