Chemi-Verse™ BLK Kinase Assay Kit

Products from BPS Bioscience require a minimum order value above 400€

Applications: Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Background: BLK (B lymphocyte kinase) is a member of the Src non-receptor tyrosine kinase family. It is found primarily in B-cells, where it is involved in cell development and maturation signaling pathways. Overexpression of BLK has been associated with autoimmune disorders and B-cell linked-cancers, such as lymphoma and leukemia. Similarly to other Src kinases, BLK is inhibited by dasatinib, a drug approved under the name SPRYCEL® for the treatment of CML (chronic myeloid leukemia) in adults and children. The development of BLK-specific inhibitors is still an active research area, aiming at finding specific and high affinity inhibitors that can be used in the treatment not only of BLK-related lymphoma and leukemia, but also autoimmune disorders.

Contraindications: The final concentration of DMSO in the assay should not exceed 1%.

Description: The Chemi-Verse™ BLK Kinase Assay Kit is designed to measure BLK (B lymphocyte kinase) kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant BLK kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed

Uniprot: P51451

Warnings: Avoid freeze/thaw cycles

Biosafety Level: Not applicable (BSL-1)

References: Yang S., et al., 2023 J Biomol Struct Dyn: 1-11.
Fu T., et al., 2022 Eur J Med Chem 229: 114051.

• Artikelnummer: BPS82229
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 82229
• Verpackungseinheit: 96 reactions
• Mengeneinheit: PAK