Products from BPS Bioscience require a minimum order value above 400€Encompassing Amino Acids: 1-261(end)
Application: Useful for for SPR analysis, animal immunization, ELISA, and PK assay.
Assay Conditions: Immobilized Human Claudin18.2 VLP at 5 µg/mL (100 µL/Well).
Background: Claudins are integral membrane proteins and major components of tight junction strands which serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. The claudin-18 gene has two splice variants: claudin 18.1, which is found primarily in the lung, and claudin 18.2, which is expressed exclusively in the stomach. In claudin 18-deficient studies, claudin 18.1 was identified as a regulator of normal lung homeostasis as its deficiency promoted the proliferative state of malignant cells found in lung adenocarcinomas.
1 Therefore, claudin-18.1 may be a potential therapeutic target in cancer.
1Biological Activity: Dose response curve for Anti-Claudin18.2 Antibody hFc with the EC
50 of ‰¤13 ng/mL determined by ELISA.
Description: Human Claudin-18 (CLDN18) protein (VLP), full length encompassing amino acids 1-261(end). This construct is tag-free and was produced using VLP (virus-like particle) technology.
Endotoxin Level: <1 EU/µg (determined by LAL method)
Format: Aqueous buffer solution
Formulation: Supplied as 0.22 µm filtered solution in PBS, pH 7.4.
Purity: ≥95% (determined by HPLC)
Storage Stability: Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Uniprot: P56856-2
Warnings: Avoid repeated freeze-thaw cycles.
Biosafety Level: BSL-1
References:
Kyuno D., Takasawa A., Kikuchi S., Takemasa I., Osanai M., Kojima T. Role of tight junctions in the epithelial-to-mesenchymal transition of cancer cells. Biochimica et Biophysica Acta (BBA) - Biomembranes, Volume 1863, Issue 3, 2021, 183503,
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