Products from BPS Bioscience require a minimum order value above 400€
Encompassing Amino Acids: 1-209(end)
Application: Useful for animal immunization and ELISA.
Assay Conditions: Measured by its binding ability in a functional ELISA.
Background: Claudin-4 mediates paracellular chloride transport and plays an important renal function through the reabsorption of filtered chloride. The CLDN4 gene is deleted in Williams-Beuren syndrome, leading to multi-systemic neurodevelopmental disorders. The protein acts as a high-affinity receptor for Clostridium perfringens enterotoxin (CPE) during infection by gram-positive Clostridial bacteria, which are responsible for food poisoning and gastrointestinal illnesses. Claudin 4 can be used as a marker for distinguishing malignant mesothelioma from lung cancer and uterine serous carcinoma. As a pancreatic cancer marker, Claudin-4 exhibits superior performance to BerEp4 staining. Claudin-4 may be overexpressed in ovarian cancer, and it accelerates cell migration and invasion in ovarian tumor cell lines.
Biological Activity: Immobilized Human CLDN4 at 5 µg/mL can bind Anti-CLDN4 recombinant antibody, the EC50 is ‰¤18 ng/mL.
Description: Human Claudin-4 (CLDN4) protein (VLP), full length encompassing amino acids 1-209(end). This construct contains a C-terminal His-tag (10xHis) and was produced using VLP (virus-like particle) technology.
Endotoxin Level: <1 EU/µg (determined by LAL method)
Format: Lyophilized powder
Formulation: Lyophilized from a 0.2 µm filtered solution of PBS, 6% Trehalose, pH 7.4.
Reconstitution: It is not recommended to reconstitute to a concentration less than 100 µg/mL in ddH2O.
Storage Stability: Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Tags: C-terminal His-tag
Uniprot: O14493
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-1
Deutsch
English
