Collagen Type II, human

Collagen Type II, human, Host: Rabbit, Polyclonal

Background: Type II collagen is an alpha1(II)-trimer, which forms 67 nm cross-banded fibrils. Typically it can be observed in cartilage and various tumours. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Human collagen type II, cross-reactivity with human collagen type I, IX and XI <0.1%.

Positive Control: Human cartilage.

Immunogen: Purified collagen type II from human cartilage.

Purification Method: affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!.

Concentration: app. 1 mg/ml.

References: 1. Guerret S., Govignon E., Hartmann D.J., Ronfard V. (2003) Long term remodeling of a bilayered living human skin equivalent (Apligraf®) grafted onto nude mice: immunolocalization of human cells and characterization of extracellular matrix. Wound Rep. Reg. 2003, 11, 35-45. 2. Freyria AM, Ronzière MC, Cortial D, Galois L, Hartmann D, Herbage D, Mallein-Gerin F. (2009) Comparative phenotypic analysis of articular chondrocytes cultured within type I or type II collagen scaffolds. Tissue Eng Part A. 2009, 15, 1233-1245. 3. Perrier E., Ronzière M.C.C., Bareille R., Pinzano A., Mallein-Gerin F., Freyria A.M (2011) Analysis of collagen expression during chondrogenic induction of human bone marrow mesenchymal stem cells. Biotechnol Lett. 2011 Oct;33(10):2091-2101 4. Claus S., Mayer N., Aubert-Foucher E., Chajra H, Perrier-Groult E., Lafond J., Piperno M., Damour O., Mallein-Gerin F.Cartilage-(2012) Characteristic Matrix Reconstruction by Sequential Addition of Soluble Factors During Expansion of Human Articular Chondrocytes and Their Cultivation in Collagen Sponges - Tissue Eng Part C Methods. 2012 Feb;18(2):104-112.

UniProt: P02458 (CO2A1_HUMAN).

Caution: *These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

• Artikelnummer: BILCO20211-0.1
• Hersteller: BioLogo
• Hersteller Artikelnummer: CO20211-0.1
• Green Labware: Nein
• Verpackungseinheit: 0,1 ml
• Mengeneinheit: STK
• Reaktivität: Human
• Klonalität: Polyclonal
• Methode: Immunofluorescence, Immunohistochemistry (paraffin), Western Blotting, ELISA, Radioimmunoassay (RIA)
• Wirt: Rabbit