Collagen Type IV, human

Collagen Type IV, human, Host: Rabbit, Polyclonal

Background: Type IV Collagen is a non-fibrillary network of different alpha-chains: alpha 1(IV) to-alpha 6(IV). It is typically found in basal membranes of different organs (e.g. skin, lens, lung, kidney). Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Human collagen type IV 100%; human collagen I, II <0.1%; human collagen III, V <1.0%; human fibronectin < 0.1%; mouse laminin < 0.1% (RIA at 1:10000 dilution).

Positive Control: Human skin, placenta or lens capsule.

Immunogen: Purified collagen type IV from human placenta.

Purification Method: Affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!.

Concentration: app. 1 mg/ml.

References: 1. Laurent R., Nallet A., Obert L., Nicod L., Gindraux F. (2014) Storage and qualification of viable intact human amniotic graft and technology transfer to a tissue bank. Cell Tissue Bank. Jun;15(2):267-75. 2. Heidet L., Cai Y., Guicharnaud L., Antignac C., Gubler M.C. (2000) Glomerular expression of type IV collagen chains in normal and X-linked alport syndrome kidneys. Am. J. Pathol 156, 1901-1910. 3. Castera L., Hartmann D.J., Chapel F., Guettier C., Mal F., Lons T., Richardet J.P., Grimbert S., Morassi O., Beaugrand M., Trinchet J.C. (2000) Serum laminin and type IV collagen are accurate markers of histologically severe alcoholic hepatitis in patient 4. Julliard A.K., Hartmann D.J. (1998) Spatiotemporal patterns of expression of extracellular matrix molecules in the developing and adult rat olfactory system. Neuroscience 84, 1135-1150.

UniProt: P02462 (CO4A1_HUMAN) ; P08572 (CO4A2_HUMAN); P29400 (CO4A5_HUMAN);.

Caution: *These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

• Artikelnummer: BILCO20411-0.1
• Hersteller: BioLogo
• Hersteller Artikelnummer: CO20411-0.1
• Green Labware: Nein
• Verpackungseinheit: 0,1 ml
• Mengeneinheit: STK
• Reaktivität: Human
• Klonalität: Polyclonal
• Methode: Immunofluorescence, Immunohistochemistry (paraffin), ELISA, Radioimmunoassay (RIA)
• Wirt: Rabbit