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Collagen Type IV, human
Collagen Type IV, human, IgG1, Host: Mouse, Monoclonal, Clone: 24D1
Artikelnummer
BILCO60411
Verpackungseinheit
0,1 ml
Hersteller
BioLogo
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Clone: 24D1.
Background: Type IV Collagen is a non-fibrillary network of different alpha-chains: alpha 1(IV) to-alpha 6(IV). It is typically found in basal membranes of different organs (e.g. skin, lens, lung, kidney). Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Human collagen type IV 100%, human collagen types I, II, III, and V <0.1%, human fibronectin and laminin < 0.1% (RIA at 1:10000 dilution).
Positive Control: Human skin, placenta or lens capsule.
Immunogen: Purified collagen type IV from human placenta.
Purification Method: 0.1 ml ProtG affinity purified antibody prod. in nude mice; lyophilized from phosphate buffered solution; no BSA and preservative added!.
Concentration: app. 0.2-0.4 mg/ml.
Source: Mouse monoclonal antibody obtained from fusion of splenocytes of animals immunized with purified collagen type IV from human placenta.
References: 1. Guerret S, Govignon E, Hartmann DJ, Ronfard V. (2003) Long-term remodeling of a bilayered living human skin equivalent (Apligraf) grafted onto nude mice: immunolocalization of human cells and characterization of extracellular matrix. Wound Repair Regen. Jan-Feb;11(1):35-452. Rousseau C., Thillou F., Pagnon-Minot A., Guerret S., Hartmann D.J, Ronfard V. (2009) Short term integration on human bilayered living skin substitute (Apligraf®) grafted onto nude mice - 19th Annual Meeting of the Wound Healing Society with SAWC (Symposium on Advanced Wound Care) 2009 April 26-29, Gaylord Texan Resort and Convention Center, Dallas, Texas.
UniProt: P02462 (CO4A1_HUMAN) ; P08572 (CO4A2_HUMAN); P29400 (CO4A5_HUMAN);.
Caution: *These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
Background: Type IV Collagen is a non-fibrillary network of different alpha-chains: alpha 1(IV) to-alpha 6(IV). It is typically found in basal membranes of different organs (e.g. skin, lens, lung, kidney). Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Human collagen type IV 100%, human collagen types I, II, III, and V <0.1%, human fibronectin and laminin < 0.1% (RIA at 1:10000 dilution).
Positive Control: Human skin, placenta or lens capsule.
Immunogen: Purified collagen type IV from human placenta.
Purification Method: 0.1 ml ProtG affinity purified antibody prod. in nude mice; lyophilized from phosphate buffered solution; no BSA and preservative added!.
Concentration: app. 0.2-0.4 mg/ml.
Source: Mouse monoclonal antibody obtained from fusion of splenocytes of animals immunized with purified collagen type IV from human placenta.
References: 1. Guerret S, Govignon E, Hartmann DJ, Ronfard V. (2003) Long-term remodeling of a bilayered living human skin equivalent (Apligraf) grafted onto nude mice: immunolocalization of human cells and characterization of extracellular matrix. Wound Repair Regen. Jan-Feb;11(1):35-452. Rousseau C., Thillou F., Pagnon-Minot A., Guerret S., Hartmann D.J, Ronfard V. (2009) Short term integration on human bilayered living skin substitute (Apligraf®) grafted onto nude mice - 19th Annual Meeting of the Wound Healing Society with SAWC (Symposium on Advanced Wound Care) 2009 April 26-29, Gaylord Texan Resort and Convention Center, Dallas, Texas.
UniProt: P02462 (CO4A1_HUMAN) ; P08572 (CO4A2_HUMAN); P29400 (CO4A5_HUMAN);.
Caution: *These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
| Artikelnummer | BILCO60411 |
|---|---|
| Hersteller | BioLogo |
| Hersteller Artikelnummer | CO60411 |
| Green Labware | Nein |
| Verpackungseinheit | 0,1 ml |
| Mengeneinheit | STK |
| Reaktivität | Human |
| Klonalität | Monoclonal |
| Methode | Immunofluorescence, Immunohistochemistry (paraffin), Western Blotting, ELISA, Radioimmunoassay (RIA) |
| Isotyp | IgG1 |
| Wirt | Mouse |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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