Collagen Type IV, porcine

Collagen Type IV, porcine, Host: Rabbit, Polyclonal

Background: Type IV Collagen is a non-fibrillary network of different alpha-chains: alpha 1(IV) to-alpha 6(IV). It is typically found in basal membranes of different organs (e.g. skin, lens, lung, kidney). Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Porcine collagen type IV 100%, porcine collagens type I, II, and III <0.1%, porcine elastin, fibronectin and laminin < 0.1% (RIA at 1:5000 dilution).

Positive Control: Porcine skin or liver.

Concentration: app. 1 mg/ml.

Source: Rabbits were immunized with purified collagen type IV from porcine kidney tissue.

References: 1. Boucard N, Viton C, Agay D, Mari E, Roger T, Chancerelle Y, Domard A ( 2007) The use of physical hydrogels of chitosan for skin regeneration following third-degree burns. Biomaterials. Aug;28(24):3478-88.

Caution: *These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

• Artikelnummer: BILCO20491-0.1
• Hersteller: BioLogo
• Hersteller Artikelnummer: CO20491-0.1
• Green Labware: Nein
• Verpackungseinheit: 0,1 ml
• Mengeneinheit: STK
• Reaktivität: Pig (Porcine)
• Klonalität: Polyclonal
• Methode: Immunofluorescence, Immunohistochemistry (paraffin), Western Blotting, ELISA, Radioimmunoassay (RIA)
• Wirt: Rabbit