Collagen Type V, human

Collagen Type V, human, Host: Rabbit, Polyclonal

Background: Collagen type V is found as short fibrils in skin, bone and placenta. Often it is found in conjunction with other collagen types, especially type I and III. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Human collagen type V 100%, human collagen type I, III and IV <0.1%, human fibronectin and laminin <0.1% (RIA at 1:100 dilution).

Positive Control: Human placenta.

Immunogen: Purified collagen type V from human placenta.

Purification Method: Affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!.

Concentration: app. 1 mg/ml.

References: 1. Laurent R., Nallet A., Obert L., Nicod L., Gindraux F. (2014) Storage and qualification of viable intact human amniotic graft and technology transfer to a tissue bank. Cell Tissue Bank. Jun;15(2):267-75. 2. Black A.F., Bouez C., Perrier E., Schlotmann K., Chapuis F., Damour O. (2005) Optimization and characterization of an Engineered human skin equivalent. Tissue Engineering 11, 723-733. 3. Chanut-Delalande H., Fichard A., Bernocco S., Garrone R., Hulmes D.J.S., Ruggiero F. (2001) Control of heterotypic fibril formation by collagen V is determined by chain stoichiometry. J. Biol. Chem. 276, 24352-24359. 4. Kleman J.P., Hartmann D.J., Ramirez F., Van der Rest M. (1992) The human rhabdomyosarcoma cell line A204 lays down a highly insoluble matrix composed mainly of 1 type-XI and 2 type-V collagen chains. Eur. J. Biochem. 210, 329-335.

UniProt: P20908 (CO5A1_HUMAN).

Caution: *These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

• Artikelnummer: BILCO20511-0.1
• Hersteller: BioLogo
• Hersteller Artikelnummer: CO20511-0.1
• Green Labware: Nein
• Verpackungseinheit: 0,1 ml
• Mengeneinheit: STK
• Reaktivität: Human
• Klonalität: Polyclonal
• Methode: Immunofluorescence, Immunohistochemistry (paraffin), Western Blotting, ELISA, Radioimmunoassay (RIA)
• Wirt: Rabbit