Corticosterone EIA Kit

Colorimetric detection of corticosterone

Target: Corticosterone

Product Type: EIA Kits

Trademark: StressXpress®

Detection Method: Colorimetric Assay

Assay Type: Sandwich EIA (Enzyme Immunoassay)

Utility: EIA kit used to measure the corticosterone present in samples.

Precision: Intra Assay Precision: Four human samples were diluted with Assay Buffer and run in replicates of 20 in an assay. The mean and precision of the calculated Corticosterone concentrations were:Sample 1- 2460.6 pg/mL, 6.3% CVSample 2- 601.5 pg/mL, 6.5% CVSample 3- 371.6 pg/mL, 3.1% CV Sample 4- 259.0 pg/mL, 4.8% CV Inter Assay Precision: Three human samples were diluted with Assay Buffer and run in duplicates in fourteen assays run over multiple days by four operators. The mean and precision of the calculated Corticosterone concentrations were:Sample 1- 2618.3 pg/mL, 7.5% CVSample 2- 630.1 pg/mL, 6.4% CVSample 3- 267.9 pg/mL, 9.9% CV

Sample Type(s): Serum,EDTA Plasma,Heparin Plasma,Urine,Tissue Culture Media,Dried Fecal Samples

Number of Samples: 39 samples in duplicate

Sensitivity: 18.6 pg/ml

Assay Range: 78.125 - 10,000 pg/ml

Scientific Background: Corticosterone (C21H30O4, Kendall's Compound ‘B') is a glucocorticoid secreted by the cortex of the adrenal gland. Corticosterone is produced in response to stimulation of the adrenal cortex by ACTH and is the precursor of aldosterone. Corticosterone is a major indicator of stress and is the major stress steroid produced in non-human mammals. Studies involving corticosterone and levels of stress include impairment of long term memory retrieval (1), chronic corticosterone elevation due to dietary restrictions (2) and in response to burn injuries (3). In addition to stress levels, corticosterone is believed to play a decisive role in sleep-wake patterns (4,5).

References: 1. Hupé, JM, et al. Nature, 1998; 394: 784-787.2. Kitaysky AS, Kitaiskaia EV, Wingfield JC, Piatt JF. J. Comp. Physiol, 2001; 171: 701-709.3. Thellin O, Noel G, Khuana S, Ogle CK and Horseman ND. Shock, 2001; 16(5): 393-397.4. Krame, KM. and Sothern RB. Chronobiol. Int., 2001; 18(6): 933-945.5. Vazquez-Palacios G, et al. Pharmacol. Biochem Behavior, 2001: 70(2-3): 305-310.

Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• Artikelnummer: STRSKT-205-96
• Hersteller: Stressmarq Biosciences
• Hersteller Artikelnummer: SKT-205-96
• Green Labware: Nein
• Verpackungseinheit: 96 well
• Mengeneinheit: PAK
• Reaktivität: Various species
• Methode: Enzyme Immunoassay