Target: Corticosterone
Product Type: EIA Kits
Trademark: StressXpress®
Detection Method: Colorimetric Assay
Assay Type: Sandwich EIA (Enzyme Immunoassay)
Utility: EIA kit used to measure the corticosterone present in samples.
Precision: Intra Assay Precision: Four human samples were diluted with Assay Buffer and run in replicates of 20 in an assay. The mean and precision of the calculated Corticosterone concentrations were:Sample 1- 2460.6 pg/mL, 6.3% CVSample 2- 601.5 pg/mL, 6.5% CVSample 3- 371.6 pg/mL, 3.1% CV Sample 4- 259.0 pg/mL, 4.8% CV Inter Assay Precision: Three human samples were diluted with Assay Buffer and run in duplicates in fourteen assays run over multiple days by four operators. The mean and precision of the calculated Corticosterone concentrations were:Sample 1- 2618.3 pg/mL, 7.5% CVSample 2- 630.1 pg/mL, 6.4% CVSample 3- 267.9 pg/mL, 9.9% CV
Sample Type(s): Serum / EDTA Plasma / Heparin Plasma / Urine / Tissue Culture Media / Dried Fecal Samples
Number of Samples: 39 samples in duplicate
Sensitivity: 18.6 pg/ml
Assay Range: 78.125 - 10,000 pg/ml
Scientific Background: Corticosterone (C21H30O4, Kendall’s Compound ‘B’) is a glucocorticoid secreted by the cortex of the adrenal gland. Corticosterone is produced in response to stimulation of the adrenal cortex by ACTH and is the precursor of aldosterone. Corticosterone is a major indicator of stress and is the major stress steroid produced in non-human mammals. Studies involving corticosterone and levels of stress include impairment of long-term memory retrieval (1), chronic corticosterone elevation due to dietary restrictions (2) and in response to burn injuries (3). In addition to stress levels, corticosterone is believed to play a decisive role in sleep-wake patterns (4,5) In a mouse model of Parkinson’s Disease, chronic corticosterone administration has been shown to exacerbate alpha synuclein pathology, leading to increased phosphorylation and the deposition of insoluble phospho-Serine129 alpha-synuclein in the hypothalamus (6). Additionally, corticosterone administration has been shown to contribute to dopaminergic neuronal loss (7).
References: 1. Hupé, J. M., James, A. C., Payne, B. R., Lomber, S. G., Girard, P., & Bullier, J. (1998). Cortical feedback improves discrimination between figure and background by V1, V2, and V3 neurons. Nature, 394(6695), 784–787. DOI: 10.1038/295552. Kitaysky, A. S., Kitaiskaia, E. V., Wingfield, J. C., & Piatt, J. F. (2001). Dietary restrictions cause chronic elevation of corticosterone and enhance stress response in red-legged kittiwake chicks. Journal of Comparative Physiology B, 171(8), 701–709. DOI: 10.1007/s0036001002303. Thellin, O., Noel, G., Khuana, S., Ogle, C. K., & Horseman, N. D. (2001). Stress hormone secretion and gut signal transducer (STAT) proteins after burn injury in rats. Shock, 16(5), 393–397. DOI: 10.1097/00024382-200116050-000014. Kramer, K. M., & Sothern, R. B. (2001). Circadian characteristics of corticosterone secretion in red-backed voles (Clethrionomys gapperi). Chronobiology International, 18(6), 933–945. DOI: 10.1081/CBI-1001075425. Vazquez-Palacios, G., Retana-Márquez, S., Bonilla-Jaime, H., & Velázquez-Moctezuma, J. (2001). Further definition of the effect of corticosterone on the sleep-wake pattern in the male rat. Pharmacology, Biochemistry, and Behavior, 70(2–3), 305–310. DOI: 10.1016/S0091-3057(01)00629-86. Bimpos, N. M., Xie, Y., Gomez-Isla, T., Domenger, D., & Hyman, B. T. (2024). Alpha-synuclein-induced stress sensitivity renders the Parkinson’s disease brain susceptible to neurodegeneration. Acta Neuropathologica Communications, 12, 100. DOI: 10.1186/s40478-024-01797-w.7. Burtscher, J., Copin, J. C., Rodrigues, J., & Pernet, N. (2019). Chronic corticosterone aggravates behavioral and neuronal symptomatology in a mouse model of alpha-synuclein pathology. Neurobiology of Aging, 83, 11–20. DOI: 10.1016/j.neurobiolaging.2019.08.011
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
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