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COX1, FLAG-His-Tags Recombinant
COX1, FLAG-His-Tags Recombinant
Artikelnummer
BPS71110-1
Verpackungseinheit
100 µg
Hersteller
BPS Bioscience
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Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Mixed various concentrations of enzyme in Assay buffer (100 mM Tris-HCl pH 8, 3 uM EDTA) with 5 µM Hematin for 10 min then added 100 M Ampliflu Red and 200 µM arachidonic acid. Fluorescence (ex. 535 em. 590) was measured after 5 minutes at room temperature. Reaction volume was 100 µl.
Description: Human COX1, GenBank Accession No. NM_000962, a.a. 1-599 (end), with C-terminal FLAG-His-tag, MW=70.5 kDa, expressed in a baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 80 µg/ml FLAG peptide, 300 µM diethyldithiocarbamate and 0.08% Triton X-100.
Genbank: NM_000962
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal FLAG-His-tags
Uniprot: P23219
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Scott, B.T., et al., Blood Coagul Fibrinolysis. 2002 Sep;13(6):519-31.
2. Funk, C.D., et al., FASEB J. 1991 Jun;5(9):2304-12.
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Mixed various concentrations of enzyme in Assay buffer (100 mM Tris-HCl pH 8, 3 uM EDTA) with 5 µM Hematin for 10 min then added 100 M Ampliflu Red and 200 µM arachidonic acid. Fluorescence (ex. 535 em. 590) was measured after 5 minutes at room temperature. Reaction volume was 100 µl.
Description: Human COX1, GenBank Accession No. NM_000962, a.a. 1-599 (end), with C-terminal FLAG-His-tag, MW=70.5 kDa, expressed in a baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 80 µg/ml FLAG peptide, 300 µM diethyldithiocarbamate and 0.08% Triton X-100.
Genbank: NM_000962
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal FLAG-His-tags
Uniprot: P23219
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Scott, B.T., et al., Blood Coagul Fibrinolysis. 2002 Sep;13(6):519-31.
2. Funk, C.D., et al., FASEB J. 1991 Jun;5(9):2304-12.
| Artikelnummer | BPS71110-1 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 71110-1 |
| Green Labware | Nein |
| Verpackungseinheit | 100 µg |
| Mengeneinheit | STK |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
