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Encompassing Amino Acids: 2-1992
Description: Human Dicer, also known as Dicer 1, Ribonuclease III, GenBank Accession No. NM_030621, a.a. 2-1992 (end), with a N-terminal His-Tag, expressed in an Sf9 cell expression system. MW =221 kDa.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 40 mM imidazole
Genbank: NM_030621
Host Cell Line: Sf9 cells
Purity: 0,2
Storage Stability: At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.
Tags: N-terminal His-Tag
Uniprot: Q9UPY3
Warnings: Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).
Biosafety Level: Not applicable (BSL-1)
References: 1.Hutagnr, G., et al. Science. 2001; 293(5531): 834-838.2.Bernstein, E., et al., Nat. Genetics. 2003; 35: 215-217 (2003).