English
ERp57 (GRP58) Antibody, Clone Map.ERp57 (GRP58): RPE
Mouse Anti-Human ERp57 (GRP58) Monoclonal IgG1
Artikelnummer
STRSMC-168B-RPE
Verpackungseinheit
200 µg
Hersteller
Stressmarq Biosciences
Verfügbarkeit:
wird geladen...
Preis wird geladen...
Target: ERp57
Conjugate: RPE
Product Type: Monoclonal
Clone Number: Map.ERp57
Immunogen: Human recombinant ERp57 (Grp58)
Swiss-Prot: P30101
Purification: Protein G Purified
Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Concentration: 1 mg/ml
Specificity: Detects ~57kDa.
Cellular Localization: Endoplasmic Reticulum,Endoplasmic Reticulum Lumen,Melanosome
Scientific Background: ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs (1-5). It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin (lectin chaperones) via their praline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glycosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the cell surface (6). ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding domain. ERp57 is induced by both estrogen and leuteinizing-hormone-releasing hormone in the hippocampus (7).
References: 1. Herbert D.N. and Molinari M. (2007) Physiol Rev. 87: 1377-1408.2. Williams D.B. (2005) J Cell Sci. 119: 615-6233. Maattanen P., et al. (2006) Biochem Cell Biol. 84: 881-889.4. Oliver J.D., et al. (1999) Mol Bio Cell. 10: 2573-2582.5. Oliver J.D., et al. (1997) Science 275: 86-88.6. Solda T., et al. (2006) J Biol Chem 281: 6219-6226.7. Kimura T., et al. (2005) Biochem Biophys Research Communications. 331 (1): 224-230.8. Chen, G., et al. (2002) Clin Cancer Res 8(7): 2298-2305.9. Tan, P., et al. F. (2002) J Immunol 168(4): 1950-1960.
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Conjugate: RPE
Product Type: Monoclonal
Clone Number: Map.ERp57
Immunogen: Human recombinant ERp57 (Grp58)
Swiss-Prot: P30101
Purification: Protein G Purified
Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Concentration: 1 mg/ml
Specificity: Detects ~57kDa.
Cellular Localization: Endoplasmic Reticulum,Endoplasmic Reticulum Lumen,Melanosome
Scientific Background: ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs (1-5). It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin (lectin chaperones) via their praline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glycosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the cell surface (6). ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding domain. ERp57 is induced by both estrogen and leuteinizing-hormone-releasing hormone in the hippocampus (7).
References: 1. Herbert D.N. and Molinari M. (2007) Physiol Rev. 87: 1377-1408.2. Williams D.B. (2005) J Cell Sci. 119: 615-6233. Maattanen P., et al. (2006) Biochem Cell Biol. 84: 881-889.4. Oliver J.D., et al. (1999) Mol Bio Cell. 10: 2573-2582.5. Oliver J.D., et al. (1997) Science 275: 86-88.6. Solda T., et al. (2006) J Biol Chem 281: 6219-6226.7. Kimura T., et al. (2005) Biochem Biophys Research Communications. 331 (1): 224-230.8. Chen, G., et al. (2002) Clin Cancer Res 8(7): 2298-2305.9. Tan, P., et al. F. (2002) J Immunol 168(4): 1950-1960.
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
| Artikelnummer | STRSMC-168B-RPE |
|---|---|
| Hersteller | Stressmarq Biosciences |
| Hersteller Artikelnummer | SMC-168B-RPE |
| Green Labware | Nein |
| Verpackungseinheit | 200 µg |
| Mengeneinheit | STK |
| Reaktivität | Human, Mouse (Murine), Rat (Rattus), Pig (Porcine), Monkey (Primate), Rabbit, Dog (Canine), Guinea Pig, Cow (Bovine), Hamster |
| Klonalität | Monoclonal |
| Methode | Immunofluorescence, Immunoprecipitation, Western Blotting, Immunohistochemistry, Immunocytochemistry |
| Isotyp | IgG1 |
| Human Gene ID | 2923 |
| Wirt | Mouse |
| Produktinformation (PDF) | Download |
| MSDS (PDF) | Download |
Verwandte Artikel
Markieren Sie die Artikel, um Sie dem Warenkorb hinzuzufügen oder
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
