FBXL11 (KDM2A) Homogeneous Assay Kit

Products from BPS Bioscience require a minimum order value above 400€

Applications: Great for studying enzyme kinetics and HTS applications.

Contraindications: Avoid green and blue dyes that absorb light in the Alphascreen^® signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN₃) or metal ions (Fe²⁺, Fe³⁺, Cu²⁺, Zn²⁺ and Ni²⁺). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect Alphascreen^® assays.

Description: The FBXL11 Homogeneous Assay Kit is designed to measure FBXL11
activity for screening and profiling applications. FBXL11, also known as KDM2A, JHDM1A, and Ndy2, is a histone lysine demethylase that exhibits demethylation activity toward H3-K₃₆Me².The key to the FBXL11 Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing FBXL11 enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

Storage Stability: At least one year from date of receipt when stored as directed.

Supplied As: The FBXL11 Homogeneous Assay Kit comes in a convenient AlphaLISA® format (Scheme 1), with biotinylated histone H3 peptide substrate, primary antibody, demethylase assay buffer, and purified FBXL11 for 96 or 384 enzyme reactions.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: Iwase, S., et al. Cell 2007; 128(6):1077-1088.

• Artikelnummer: BPS50611-1
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 50611-1
• Green Labware: Nein
• Verpackungseinheit: 96 rxns.
• Mengeneinheit: PAK