Goat anti Monkey IgA (Fc specific), conjugated with Horseradish peroxidase

Goat anti Monkey IgA (Fc specific), conjugated with Horseradish peroxidase, Polyclonal, Clone: Polyclonal

Clone: Polyclonal

Label: HRP

Background: The reactivity of the antiserum is directed to the Fc subunit of the IgA molecule which expresses strict isotypic (class) specificity. It does not react with any non-Ig protein in monkey serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgA at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of monkey origin known to be of the IgA isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgA in monkey serum or other body fluids. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA.This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Source: Purified normal IgA isolated from pooled rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Specificity: Horseradish peroxidase-conjugated IgG fraction of polyclonal Goat antiSerum to Monkey IgA, Fc specific.

Formulation: Horseradish peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.

References: Ols S, Yang L, Thompson EA, Pushparaj P, Tran K, Liang F, Lin A, Eriksson B, Karlsson Hedestam GB, Wyatt RT, Loré K. Route of Vaccine Administration Alters Antigen Trafficking but Not Innate or Adaptive Immunity. Cell Rep. 2020 Mar 24;30(12):3964-3971.e7. doi: 10.1016/j.celrep.2020.02.111.Sundling C, Schön K, Mörner A, Forsell MNE, Wyatt RT, Thorstensson R, Hedestam GBK, Lycke NY. CTA1-DD adjuvant promotes strong immunity against human immunodeficiency virus type 1 envelope glycoproteins following mucosal immunization.J Gen Virol. 2008 Dec;89(Pt 12):2954-2964. doi: 10.1099/vir.0.2008/005470-0

Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

• Artikelnummer: GAMon/IgAFcPO
• Hersteller: Nordic-MUbio
• Hersteller Artikelnummer: GAMon/IgA(Fc)/PO
• Green Labware: Nein
• Verpackungseinheit: 1 ml
• Mengeneinheit: STK
• Reaktivität: Monkey (Primate)
• Klonalität: Polyclonal
• Methode: ELISA, Immunohistochemistry, Immunocytochemistry, Dot Blot, Immunoblotting
• Wirt: Goat