Goat anti Monkey IgG (Fc specific), conjugated with Horseradish peroxidase

Goat anti Monkey IgG (Fc specific), conjugated with Horseradish peroxidase, Polyclonal, Clone: Polyclonal
Artikelnummer
GAMon/IgGFcPO
Verpackungseinheit
1 ml
Hersteller
Nordic-MUbio

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Clone: Polyclonal

Background: The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule which expresses strict isotypic (class) specificity. It does not react with any non-Ig protein in monkey serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of monkey origin known to be of the IgG isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG in monkey serum or other body fluids.This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Source: Purified normal IgG isolated from pooled rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Specificity: Horseradish peroxidase-conjugated IgG fraction of polyclonal Goat antiSerum to Monkey IgG, Fc specific.

Formulation: Horseradish peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.

References: 1. Sundling C, Zhang Z, Phad GE, Sheng Z, Wang Y, Mascola JR, Li Y, Wyatt RT, Shapiro L, Karlsson Hedestam GB. Single-cell and deep sequencing of IgG-switched macaque B cells reveal a diverse Ig repertoire following immunization. J Immunol. 2014 Apr 15;192(8):3637-44. doi: 10.4049/jimmunol.1303334. Epub 2014 Mar 12. doi: 10.4049/jimmunol.1303334

Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Mehr Informationen
Artikelnummer GAMon/IgGFcPO
Hersteller Nordic-MUbio
Hersteller Artikelnummer GAMon/IgG(Fc)/PO
Green Labware Nein
Verpackungseinheit 1 ml
Mengeneinheit STK
Reaktivität Monkey (Primate)
Klonalität Polyclonal
Methode Immunohistochemistry (paraffin), Western Blotting, ELISA, Immunocytochemistry, Dot Blot
Wirt Goat
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