Goat anti Monkey IgG IgA IgM (Fc specific), conjugated with Biotin

Goat anti Monkey IgG IgA IgM (Fc specific), conjugated with Biotin, Polyclonal, Clone: Polyclonal
Artikelnummer
GAMon/IgFc/Bi
Verpackungseinheit
1 ml
Hersteller
Nordic-MUbio

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Clone: Polyclonal

Background: The reactivity of the antiserum is directed to the Fc subunit of the major immunoglobulin isotypes which expresses strict isotypic (class) specificity. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal monkey plasma and serum, the characteristic IgG, IgA and IgM precipitin lines are obtained. In immunocytochemical and immunohistochemical staining of immunoglobulins at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of monkey origin in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure immunoglobulins in monkey serum or other body fluids. The absence of activity to the common Ig/Fab subunit prevents the reaction of this conjugate with immunoglobulins bounds to Fc receptors on non-lymphoid cells. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used.This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Source: Purified normal IgG , IgA and IgM isolated from pooled rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Specificity: Biotin-conjugated IgG fraction of polyclonal Goat antiSerum to Monkey IgG, IgA and IgM, Fc specific.

Formulation: Biotin-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.

Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Mehr Informationen
Artikelnummer GAMon/IgFc/Bi
Hersteller Nordic-MUbio
Hersteller Artikelnummer GAMon/Ig(Fc)/Bio
Green Labware Nein
Verpackungseinheit 1 ml
Mengeneinheit STK
Reaktivität Monkey (Primate)
Klonalität Polyclonal
Methode Immunohistochemistry (paraffin), Western Blotting, ELISA, Immunocytochemistry, Dot Blot
Wirt Goat
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