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Assay Conditions: This protein was tested at multiple concentrations with Glutathione Peroxidase Assay Kit (Cayman Chemical #703102).
Description: Recombinant active human GPX4 (glutathione peroxidase 4), encompassing amino acids 1-17-(end). This construct contains an N-terminal His-tag. The recombinant protein was affinity purified.
Format: Aqueous Solution
Formulation: 50 mM potassium phosphate buffer, pH 7.6, with 0.1 mM DTT and 5% glycerol
Purity: ≥65% estimated by SDS-PAGE
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-Tag
Uniprot: P36969-2
Unit Definition: One unit is defined as the amount of enzyme required to produce 1 nmol of NADP+ per minute at 25°C in 50 mM Tris-HCl, pH 7.6, with 5 mM EDTA, 1 mM GSH, 0.076 units glutathione reductase, 263 µM NADPH and 0.5 mM of cumene hydroperoxide.
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
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