HDEL Antibody, Clone 2E7: Biotin

Mouse Anti-Yeast HDEL Monoclonal IgG2b

Target: HDEL

Conjugate: Biotin

Product Type: Monoclonal

Clone Number: 2E7

Immunogen: Raised against a synthetic HDEL peptide corresponding to the C-terminus of yeast Bip

Purification: Protein G Purified

Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated

Concentration: 1 mg/ml

Specificity: Detects ~78kDa.

Cellular Localization: Endoplasmic Reticulum

Scientific Background: HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. They act as molecular chaperones, facilitating the assembly of multi-protein complexes; participate in the translocation of polypeptides across cell membranes and to the nucleus; and aid in the proper folding of nascent polypeptide chains (1, 2). GRP 78 is localized in the endoplasmic reticulum (ER), where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains (2). Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually KDEL in animal cells, and HDEL in S.cerevisiae (3). The 2E7 clone recognizes the C-terminal peptide HDEL, a common version of the endoplasmic reticulum retention signal found in yeast, plant, nematode and other ER proteins. 2E7 specifically stains HDEL proteins in barnyard grass, beet, cotton, mung bean, sorghum and wheat (4).

References: 1. Mayer M.P., and Bukau B. (2005) Cell Mol Life Sci. 62(6): 670-684.2. Luo S., Mao C., Lee B., and Lee A.S. (2006) Mol Cell Biol. 26(15): 5688-5697.3. Entrez Gene: HDEL, Gene ID: 109454. Napier R.M., et al. (1992) J Cell Sci. 102: 261-271.

Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• Artikelnummer: STRSMC-175D-BI
• Hersteller: Stressmarq Biosciences
• Hersteller Artikelnummer: SMC-175D-BI
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK
• Reaktivität: Yeast, Plant, Fruit Fly (Drosophila Melanogaster)
• Klonalität: Monoclonal
• Methode: Immunofluorescence, Western Blotting, Immunocytochemistry
• Isotyp: IgG2b
• Wirt: Mouse