HLA-C*08:02 K562 Cell Line

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Applications: Useful for studying antigen presentation by HLA-C.Useful for studying HLA-C restricted T cell responses.

Background: Human Leukocyte Antigen-C (HLA-C) is an MHC-I (major histocompatibility complex) heavy chain receptor, composed of HLA-C and β2-microglobulin (B2M). HLA-C is present in all cells and exists as several haplotypes due to the diversity of HLA-C genes. C*08:02 represents one such haplotype. HLA class I present neoantigen-derived peptides to the cell surface, allowing them to be recognized by T cells, via TCR (T cell receptors). Cancer immunotherapy has been taking advantage of that mechanism, by engineering T cells to express TCRs able to recognize specific cancer immunogens. In 2016 the use of HLA-C*08:02-restricted TIL (tumor infiltrating lymphocytes) targeting specifically KRAS (Kirsten rat sarcoma virus) G12D mutation in lung cancer resulted in positive results. A similar approach was pursued in a patient with metastatic pancreatic cancer and resulted in regression of the disease. The study of HLA-C*08:02-restricted TIL expressing TCR against other neoantigens may prove beneficial in cancer therapy. K562 cells are HLA class I and II negative, making them an ideal cellular model to introduce and study specific haplotype responses.

Description: The HLA-C*08:02 K562 Cell Line is an engineered human lymphoblast K562 cell line expressing human HLA-C*08:02 driven by an EF1a promoter. This cell line was generated by transduction with HLA-C*08:02 Lentivirus (BPS Bioscience #78930).This cell line has been validated by flow cytometry and co-culture assays with Jurkat cells expressing KRAS G12D, in the presence of wild type and mutant KRAS peptides

Host Cell Line: K562

Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.

Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at support@bpsbioscience.com if the cells are not frozen in dry ice upon arrival.

Supplied As: Each vial contains ˃1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Warnings: Avoid freeze/thaw cycles

Biosafety Level: BSL-1

References: Leidner R., et al., 2022 N Engl J Med 386:2112-2119.
Tran E., et al., 2016 N Eng J Med 375:2255-2262.

• Artikelnummer: BPS78974
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 78974
• Verpackungseinheit: 2 vials
• Mengeneinheit: PAK
• Wirt: Human