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Applications: Study enzyme kinetics.Screen small molecular inhibitors for drug discovery and high throughput (HTS) applications.
Background: HSP70 (Heat Shock 70kDa protein) family members are protein chaperones that play an essential role in cellular protein metabolism by acting as polypeptide-binding and release factors. These chaperones ensure the folding of newly synthesized proteins, the formation of dissociation of protein complexes, and protect against stress. HSP40 co-chaperone proteins regulate complex formation between HSP70 and client proteins. Specifically, HSP40 proteins regulate the ATP-dependent binding of HSP70 to polypeptides through their J-domain. The J-domain of HSP40 is thought to interact with HSP70 at an acidic groove located in the ATPase domain. J-domain-HSP70 complex formation plays a critical role in the regulation of HSP70 ATPase activity.
Description: The HSP70 Assay Kit is a chemiluminescence assay kit designed to measure HSP70 adenosine triphosphate (ATP) hydrolysis activity for screening and profiling applications using ADP-Glo® Assay as a detection reagent.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Supplied As: The HSP70 Assay Kit comes in a convenient 96-well format, with enough purified HSP40 and HSP70 proteins, ATP, and HSP70 assay buffer for 100 enzyme reactions.
Uniprot: P0DMV8
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)