Human beta Defensin-3 Recombinant

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Encompassing Amino Acids: 23-67

Amino Acid Sequence: GIINTLQKYYCRVRGGRCAVLSCLPKEEQIGKCSTRGRKCCRRKK

Background: Defensins are a large family of peptides of which two groups exist in mammals: alpha defensins and beta defensins, which are distinguishable by the spacing and connectivity of the conserved cysteine residues within the mature peptides. It is thought that defensins function in the eradication of pathogens from the host system by inserting themselves into the bacterial membrane under the influence of membrane potential, forming channels which lead to leakage of cytoplasmic molecules and cell death.

Biological Activity: The ED₅₀ was determined by its antimicrobial resistance to E. coli and was determined to be <3 µg/ml.

Description: Recombinant beta Defensin-3 is a disulfide-linked monomer protein consisting of 46 amino acid residues, and migrates as an approximately 5 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human beta Defensin-3 mature chain was expressed in E. coli.

Endotoxin Level: <0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

Format: lyophilized protein

Formulation: Lyophilized from 0.2 µm filtered 100 mM NaCl, 20 mM phosphate buffer, pH 7.4.

Genbank: P81534

Purity: ≥98% by SDS-PAGE and HPLC

Reconstitution: Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.

Storage Stability: The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.

Uniprot: P81534

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Antimicrob. Agents Chemother., May 2008, 52: 1876 - 1879.
2. Infect. Immun., May 2008, 76: 2169 - 2176.
3. J. Biol. Chem., Mar 2008, 283: 6631 - 6639.

• Artikelnummer: BPS90108-A
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 90108-A
• Verpackungseinheit: 5 µg
• Mengeneinheit: STK