Human Eotaxin-1 (CCL11 ) Recombinant

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Encompassing Amino Acids: 24-97

Amino Acid Sequence: GPASVPT TCCFNLANRK IPLQRLESYR RITSGKCPQK AVIFKTKLAK DICADPKKKW VQDSMKYLDQ KSPTPKP

Background: Eotaxin belongs to the platelet factor-4 family of chemokines. The gene symbol is SCYA11. The factor is being referred to also as Eotaxin-1 and has been renamed CCL11. Eotaxin induces substantial accumulation of eosinophils in the skin without significantly affecting the accumulation of neutrophils. Eotaxin is a potent stimulator of both guinea pig and human eosinophils in vitro. The Eotaxin receptor is a G-protein-coupled receptor selectively expressed in human eosinophils .

Biological Activity: Biological activity was determined by the ability to induce chemotaxis of purified eosinophils at concentrations ranging between 50-100 ng/ml.

Description: Recombinant Eotaxin-1 is a disulfide-linked homodimeric protein consisting of 75 amino acid residues, and migrates as an approximately 9 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding human Eotaxin-1 mature chain was expressed in E. coli.

Endotoxin Level: <0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

Format: lyophilized protein

Formulation: Lyophilized from 0.2 µm filtered solution (1 mg/ml) in PBS, pH 7.4.

Genbank: P51671

Purity: ≥97% by SDS-PAGE and HPLC

Reconstitution: Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.

Storage Stability: The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.

Uniprot: P51671

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Clin. Cancer Res., Apr 2009, 15: 2647 - 2656.
2. Int. Immunol., Feb 2009, 21: 187 - 201.
3. J. Immunol., Nov 2008, 181: 7390 - 7399.

• Artikelnummer: BPS90127-B
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 90127-B
• Verpackungseinheit: 20 µg
• Mengeneinheit: STK