Human Interleukin-15 Recombinant

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Encompassing Amino Acids: 49-162

Amino Acid Sequence: NW VNVISDLKKI EDLIQSMHID ATLYTESDVH PSCKVTAMKC FLLELQVISL ESGDASIHDT VENLIILANN SLSSNGNVTE SGCKECEELE EKNIKEFLQS FVHIVQMFIN TS

Background: IL-15 has been shown to be produced by human fetal astrocytes and microglial cells in response to IL-1beta, IFN-gamma, or TNF- alpha and may thus play a role in T-cell mediated immune responses in the human central nervous system. IL-15 stimulates proliferation of the established T-cell line CTLL-2, CD8(+) memory T- cells require IL-15 for proliferation. IL-15 is also able to induce generation of cytolytic cells and LAK cells activity in vitro. IL-15 appears to function as a specific maturation factor for NK-cells and can mimic the bone marrow microenvironment in vitro, which is required for NK- cells to acquire lytic functions. IL-15 induces proliferation of mast cells in the absence of functional IL-2 receptor components and utilizes a receptor of 60-65 kDa that is distinct from known components of T-cell IL-15 receptors. The IL-15 receptor on mast cells recruits JAK2 and STAT5, instead of JAK1, JAK3, and STAT3 and STAT5 that are activated in T-cells. The alpha subunit of the IL-15 receptor (IL-15RA) is structurally related to the alpha subunit of the IL-2 receptor and confers high affinity binding of IL-15 to its receptor. IL-15 binds to the beta and gamma chains of the IL2 receptor, which are known to be required for ligand internalization and signal transduction.

Description: Recombinant Interleukin-15 is a disulfide-linked homodimeric protein consisting of two 115 amino acid residues, and migrates as an approximately 13 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding human Interleukin-15 mature chain was expressed in E. coli.

Format: aqueous buffer solution

Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.

Genbank: P40933

Purity: ≥90% by SDS-PAGE

Storage Stability: Stable for at least 6 monthsfrom date of receipt when stored at -80°C. Avoid repeated freeze/thaw cycles.

Uniprot: P40933

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. J. Immunol., Sep 2009, 183: 3574 - 3577.
2. PNAS, Sep 2009, 106: 15849 - 15854.
3. Mol. Cancer Ther., Sep 2009, 8: 2736 - 2745.

• Artikelnummer: BPS90180-A
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 90180-A
• Verpackungseinheit: 2 µg
• Mengeneinheit: STK